RNase Inhibitor

RNase inhibitor was purified from human placenta, and this enzyme has a function to inhibit ribonuclease by forming a complex with RNase A. However, since this reaction is reversible, the complex is disassociated by the urea or sulfohydryl reagent. Thereby ribonuclease activity can be activated and the RNase inhibitor is irreversibly inactivated. In addition, unlike conventional antagonists (nucleotides, inorganic phosphorus acids), it can be easily removed from the reaction system by phenol treatment because it is proteinaceous.