The PCRquick-spinTM Kit is designed to purify products directly from a polymerase chain reaction. PCR products are commonly purified to remove nucleotides, dNTPs, enzymes, primers, mineral oil, salts, Etidium bromide, dyes, detergents and other impurities using the silica-membrane technology. In addition, silica-gel membrane can bind up to 15 ㎍ DNA in high-salt buffer and elute the DNA in low-salt buffer. The procedure is very simple, just bind-washelute step within <15 minutes. Purified DNA is ready for immediate use in most applications such as automated and manual DNA sequencing, nested PCR amplification, DNA labeling, restriction analysis, ligation and cloning. (Successfully purify DNA fragments from 50 bp to 10 kb, proven purity made possible using silica-based technology.)
- High yield and quality.
- Saving the time - takes only 20 minutes to extract DNA fragment from agarose gel.
- Minimizes DNA loss – recovers DNA fragment from agarose gel without solvent extraction.
- Precipitation or other steps that can lead to lost or degradation of DNA.
- Lab safety – does no use hazardous organic solvent.
Format: Spin column with 2 ml collection tubes
Column capacity: 800 ㎕
DNA binding capacity: 40 ㎍
Throughput: 1-24 samples
Prep. time: <30 minutes
Recovery: 90-95% depending on elution volume
Elution volume: 20-50 ㎕