Descripción
Kit Contents
| Contents | Description | Volumes |
|---|---|---|
| Lysis Buffer¹ | 35ml | |
| Binding Buffer | 35ml | |
| Washing Buffer A | 30ml | |
| Washing Buffer B² | Add 40 ml of EtOH before use | 10ml |
| Elution Buffer | 20ml | |
| Spin Columns | Inserted into a collection tubes. (2.0 ml tubes) |
50 columns |
| Instruction Manual | 1 sheet |
1. Lysis Buffer is composed high concentration of chaotropic salt. Carefully handle it.
2. Washing Buffer B is supplied as concentrates. Add 40 ml of ethanol (96~100 %) according to the bottle label before use.
Technical Data
Examples of amplification after extractions from various pathogenic samples
After diluting 1/10 of various pathogenic samples, total DNA / RNA was extracted with Patho Gene-spin™ DNA/RNA Extraction Kit. Sensitivity activity and amplification rates were checked using Maxime™ PCR PreMix Kit (i-StarTaq) or Maxime™ RT-PCR PreMix Kit.
Observation of amplification efficiency on various PCR/RT-PCR conditions
Total RNA was extracted from pathogenic samples using the Patho Gene-spin™ DNA/RNA Extraction Kit and competitors DNA/RNA extraction products. PCR / RT-PCR amplification with extracted samples shows that the Patho Gene-spin™ DNA/RNA Extraction Kit is suitable for stable amplification.
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