i-Taq™ Plus DNA Polymerase with Buffer, dNTPs.

i-Taq™ Plus PCR core Kit for reproducible DNA amplification and specialized PCR.

En existencia

SKU: DL0047
Categoría:

Descripción

i-Taq™ Plus PCR core Kit for reproducible DNA amplification and specialized PCR.

  • Thermostable DNA polymerase in 94 Kda.
  • Taq DNA polymerase in high purity.
  • Removal of protein originated from E.coli and DNA as PCR contaminant source.
  • Optimized buffer to highly activate polymerase regardless the sort of template and reaction condition.
  • High reproducibility.
  • Various applications for cloned DNA and human genomic DNA, etc.

i-Taq Plus DNA Polymerase, 250 units, 10x PCR Buffer (with Mg+2), 10x PCR Buffer (w/o Mg+2), 10 mM dNTPs, 25 mM Mg+2

i-Taq™ DNA Polymerase included in the product is the 94KDa thermostable DNA polymerase which is expressed in E.coli after cloning the polymerase gene of Thermus aquaticus(strain TY1). This removes protein originated from E.coli and DAN which can affect on PCR as contaminants to perform stable and effective DNA amplification and amplifies to maximum 5 Kb with genomic DNA or cDNA, etc.

i-Taq™ Plus DNA Polymerse is highly purified using a proprietary procedure that virtually eliminates contaminating bacterial DNA. The enzyme provides a thermostability that meets the requirements of specialized PCR applications. A thermostable DNA polymerase exhibits very high activity in primer extension and other molecular biology application.

The product has the best polymerase activity and high reproducibility regardless of template or reaction condition by optimizing the composition of buffer.

The PCR products can be directly used for cloning of T-Vector and blunt-end vector after making blunt end.

Applications

Amplification of Genomic DNA, cDNA up to 5 kb
T/A vector cloning
Site directed mutagenesis etc.

Información adicional

Cat.

25152

Cap.

500 units

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