Spin type product for extracting high yield and high purified DNA from various specimen such as Whole Blood, Cell, Tissue, Fixed tissue and Gram (-) Bacteria.
- Possible to extract DNA from various specimens such as Whole Blood, Cell, Tissue, Fixed tissue and Gram (-) Bacteria
- Possible to extract high yield and purified DNA with upgraded Lysis Buffer and Binding Buffer
- In case for extracting specimen from Blood, DNA can be extracted with only one buffer without performing RBC lysis separately.
- Genomic DNA extraction can be done quickly within 20~30 minutes.
- Optimized protocol for each specimen is provided.
G-spin™ Total DNA Extraction Mini Kit is instructed to extract genomic DNA simply and quickly from animal culture cell, blood, animal tissue, Rodent tail, fixed tissue, hair and Gram negative bacteria. This product provides upgraded Lysis Buffer and Binding Buffer compare to conventional G-spin™ Series in order to extract high yield and purified DNA from various specimens but also make possible to extract from blood without any process needed for RBC lysis. Also, it removes inhibitor in order to be used in downstream applications such as PCR and hybridization research. Also, it provides optimized protocol for each specimens (Table 1. Reference), therefore there is very high reproducibility for test results and expedite experiments. G-spin™ Total DNA Extraction Mini Kit verified its performance through testing various types of specimens.
Table 1. Protocols of G-spinTM Total DNA Extraction Mini Kit
|Contents||50 Columns||200 Columns|
|Buffer CL||25ml × 1 ea||90ml × 1 ea|
|Buffer BL||25ml × 1 ea||90ml × 1 ea|
|Buffer WA||40ml × 1 ea||160ml × 1 ea|
|Buffer WB (Concentration)||10ml / 5ml||40ml / 20ml|
|Buffer CE||20ml × 1 ea||40ml × 1 ea|
|Spin Column (Green color) & Collection tube||50 ea||200 ea|
|RNase A, lyophilized||3 mg x 1 vial||3 mg x 4 vial|
|Proteinase K, lyophilized||22 mg x 1 vial||22 mg x 4 vial|
|Manual||1 ea||1 ea|
Recovery rates based on various specimens
After extracting DNA from various types of samples using G-spinTM Total Genomic DNA Extraction kit, high yield and purified DNA is identified from animal cell, blood, tissue, gram-negative bacteria and biological swab.
After loading 100ng of extracted DNA using G-spinTM Total Genomic DNA Extraction Mini Kit on 0.8 % agarose gel(including 1X RedSafe), intact genomic DNA is identified.
Lane M, DNA Marker, lane 1, K562 cell; lane 2, SNU-1 cell; lane 3, U937 cell; lane 4, HeLa cell; lane 5, NIH/3T3 cell; lane 6, Vero cell; lane 7, B16 cell; lane 8, Liver(mouse); lane 9, Heart (mouse); lane 10, Lung(mouse); lane 11, Brain(mouse); lane 12, Kidney(mouse); lane 13, Spleen(mouse); lane 14, muscle(mouse); lane 15, stomach(mouse); lane 16, Tail(mouse); lane 17, Hair(human); lane 18, blood(treated EDTA); lane 19, blood(treated Heparin); lane 20, blood(treated citrate); lane 21, Buffy coat 1; lane 22, Buffy coat 2; lane 23, E. coli; lane 24, P. aeruginosa; lane 25, S. gallinarium; lane 26, Blood swab; lane 27, Buccal swab
DNA extraction Test regarding to Blood sample
In order to observe efficiency of genomic DNA extraction from blood, comparison data between competitors was obtained. Purity of extracted DNA was good when DNA was extracted from 6.25 μl to 200ul of blood volume. Efficiency of DNA extraction was excellent.
After observing DNA’s recovery rate by repeating DNA extraction third time from blood samples obtained by five different contributors, although there is discrepancy between blood contributors, stable recovery of DNA with high reproducibility was identified.