Spin column type product for quick and easy separation/purification of high yield of Plasmid DNA
- Preparation time except Cell harvest takes only 10 minutes.
- Possible to extract high purified and yield of plasmid DNA using relatively low amounts of buffer.
- Ice incubation, centrifugation are not necessary.
- Able to use extracted plasmid DNA in downstream application.
Fast DNA-spinTM Plasmid DNA Purification Kit is used for purifying and separating high yield of plasmid DNA from E.Coli using silica membrane and optimized alkali lysis buffer system. Especially, experiment time has been minimized by simplifying procedure and ice incubation, centrifugation is not necessary. This product can be stored at RT for 1 year if buffer contents including Rnase A were not used. Also, Lysis & Neutralization indicator such as Lysis Viewer is included and able to verify pH change visually in real time. Purified plasmid DNA from this kit can be used for transfection, ligation, transformation, sequencing, etc. This product is suitable for PCR and restriction enzyme experiments as well.
|Contents||50 prep||200 prep|
|M1 Buffer (Resuspension buffer)||15ml||60ml|
|M2 Buffer (Lysis buffer)||15ml||60ml|
|M3 Buffer (Neutralization buffer)||20ml||80ml|
|RNase A (10 mg/ml)||150µl||600µl|
|Spin Column||50 ea||200 ea|
|Collection Tube||50 ea||200 ea|
|Manual||1 ea||1 ea|