Fast DNA-spin™ Plasmid DNA Purification Kit

Spin column type product for quick and easy separation/purification of high yield of Plasmid DNA.

En existencia

SKU: DL0025
Categoría:

Descripción

Spin column type product for quick and easy separation/purification of high yield of Plasmid DNA

  • Preparation time except Cell harvest takes only 10 minutes.
  • Possible to extract high purified and yield of plasmid DNA using relatively low amounts of buffer.
  • Ice incubation, centrifugation are not necessary.
  • Able to use extracted plasmid DNA in downstream application.

 
Fast DNA-spinTM Plasmid DNA Purification Kit is used for purifying and separating high yield of plasmid DNA from E.Coli using silica membrane and optimized alkali lysis buffer system. Especially, experiment time has been minimized by simplifying procedure and ice incubation, centrifugation is not necessary. This product can be stored at RT for 1 year if buffer contents including Rnase A were not used. Also, Lysis & Neutralization indicator such as Lysis Viewer is included and able to verify pH change visually in real time. Purified plasmid DNA from this kit can be used for transfection, ligation, transformation, sequencing, etc. This product is suitable for PCR and restriction enzyme experiments as well.
 
 

Applications

Restriction enzyme digestion
Sequencing
Library screening
Ligation and transformation
in vitro translation
Transfection of robust cells etc.

Kit Contents

Contents 50 prep 200 prep
M1 Buffer (Resuspension buffer) 15ml 60ml
M2 Buffer (Lysis buffer) 15ml 60ml
M3 Buffer (Neutralization buffer) 20ml 80ml
Washing Buffer 15ml 50ml
Elution Buffer 15ml 30ml
Lysis Viewer 75µl 300µl
RNase A (10 mg/ml) 150µl 600µl
Spin Column 50 ea 200 ea
Collection Tube 50 ea 200 ea
Manual 1 ea 1 ea

 

Información adicional

Cat.

17095

Cap.

50 col.

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