Spin type product which can extract high purity and yield total RNA without any genomic DNA contamination easily and quickly from cells and tissues.
- A combination product of phenol solution and spin column.
- Minimization of genomic DNA contamination.
- High yield and high purity total RNA extraction.
- Unnecessary of Alcohol PPT procedure because of using column to extract total RNA.
- Highly reproducible results with the use of column.
- RNA extraction within 30 minutes.
easy-spin™ (DNA free) Total RNA Extraction Kit is designed to extract total RNA from cell and tissue in easy and fast way without mixing of genomic DNA. RNA extraction product is normally divided into column type and solution type. Solution type (phenol use) has higher yield comparing to spin type and also can extract large amount of RNA. Even though alcohol precipitation step is required due to the use of phenol, it has been widely used to extract RNA. On the contrary, spin type does not required alcohol precipitation to extract pure RNA, but it is mainly used for the rapid extraction of RNA from many samples, or for the safety and eco-friendliness of experimenters and the ease of experimentation.
As described above, the RNA extracted in the above two forms should have enough purity and yield to be used in almost all molecular biological experiments such as Northern blot analysis, cDNA synthesis and RT-PCR. It is a easy-spin™ (DNA free) Total RNA Extraction Kit that combines the advantages of the solution type and the column type. This product eliminates the inconvenience of the alcohol PPT stage when using the solution type product, and total RNA can be extracted within 30 minutes without contamination of genomic DNA. This product significantly reduces contamination concerns by using the CAPS method to individually pack the columns to effectively perform pathogen research (see Figure 1).
[ Figure 1. Spin Column & CAPS Information ]
|Lysis Buffer(easy-BLUETM Solution)||50ml × 1 ea|
|Binding Buffer||20ml × 1 ea|
|Washing Buffer A||40ml × 1 ea|
|Washing Buffer B(Concentration)||10ml × 1 ea|
|Elution Buffer||20ml × 1 ea|
|Spin Columns(Orange color)||50 Columns|
|Collection Tubes||50 tubes|
Comparison of extraction efficiency of total RNA according to various product groups
Total RNA was isolated and purified from SNU-1 cells using the easy-spin™ Total RNA Extraction Kit and competitors products; solution type and column type. In the case of extraction using the easy-spin™ Total RNA Extraction Kit, we could see that the extracted total RNA was superior to the total RNA extracted by the competitors products.
Lane M; marker; lane 1, easy-spin™ Kit; lane 2, supplier A(phenol type); lane 3, supplier B(column type)
Results of genomic DNA contamination
We confirmed the presence of gDNA contamination by PCR amplification using IL-10 primer and total RNA extracted from easy-spin™ Total RNA Extraction Kit and competitors products; solution type and column type. In the case of the competitors products, gDNA contamination was confirmed in phenol type and column type, while we could confirm that gDNA contamination was negative in the RNA extracted by the easy-spin™ Total RNA Extraction Kit.
Lane M; marker; lane 1, easy-spin™ Kit; lane 2, supplier A(column type); lane 3, supplier B (phenol type); lane P, control template gDNA