Solution type product that can easily extract high yield and purity total RNA from cell, tissue and plant etc.
- Total RNA extraction from solid samples such as cell, tissue and plant etc.
- Easy separation of aqueous phase/ phenol phase with deep-cyan blue color solution.
- High efficiency and purity total RNA extraction using high purity water-saturated phenol.
- Optimized protocol for each specimen.
- Completely eliminates cross-contaminants for precise downstream applications.
easy-BLUE™ Total RNA Extraction Kit is a solution type that can extract total RNA easily and quickly from solid samples such as cell, tissue, and plant etc. In order to efficiently extract RNA from cells and tissues, it is necessary to effectively dissolve the cells or tissues and denature nucleic acid proteins, also inhibit the activity of RNase from cells. From this point of view, the easy-BLUE™ Total RNA Extraction Kit is the product that can most efficiently extract the RNA from tissues or cells. It is theoretically possible to extract pure RNA with almost no contamination of protein and genomic DNA when the amount of RNA extraction sample is appropriate.
In addition, the easy-BLUE™ Solution is able to acquire high yield and purity total RNA using high purity water-saturated phenol, and provides optimized protocol for each sample (see Table 1), making it easy and convenient for anyone to get results reproducibly. Total RNA obtained from this product can be directly used for cDNA synthesis, RT-PCR, Real-time RT-PCR, Northern blotting and so on.
[ Table 1. Protocols of easy-BLUE™ Total RNA Extraction Kit ]
|easy-BLUE™ Solution||100ml × 1 ea|
Total RNA extraction from various tissues and cells
RNA was extracted from various cells and tissues using the easy-BLUE™ Total RNA Extraction Kit. As results, it was confirmed that high-purity RNA could be extracted.
Lane M, 1 Kb Marker; lane 1, SNU 601(Human); lane 2, K562(Human); lane 3, B16(Mouse); lane 4, E. coli; lane 5, Spleen(Mouse); lane 6, Kidney(Mouse); lane 7, Liver (Mouse)
Comparison of quality with other products
RNA was extracted from various cells and tissues using the easy-BLUE ™ Total RNA Extraction Kit. As results,
its extraction efficiency showed equal to or better than other products.