Double-RNA Viral dsRNA Extraction Mini Kit (for Plant Tissue)

Spin type product for extracting viroid, pathogenic plant virus in a short period.

En existencia

SKU: DL0036
Categoría:

Descripción

Spin type product for extracting viroid, pathogenic plant virus in a short period

  • Possible to extract viroid, pathogenic plant virus selectively within 30 minutes.
  • Possible to purify dsRNA selectively.
  • CsCl gradient, LiCl alcohol PPT are not necessary.
  • Useful for molecular diagnostic due to minimizing concerns for contamination by using CAPS (Column) method.

Double-RNA Viral dsRNA Extraction Mini Kit(For Plant Tissue)is designed to extract only double-stranded virus RNA that are 10% among plant disease infectious virus. Most plant pathogenic viruses contain single-strand RNA but it will change form to double-strand RNA during replication. These dsRNA usually binds to cellulose under low alcohol concentration. Gene extraction under low concentration of alcohol can lead to a large amount of pathogenic ssRNA based on high concentration of salt with binding Silica- based gene and a co-precipitation may occur depending on the type and amount of the sample. Therefore, this product is possible for selective dsRNA extraction in a short time because it can maximize the reproducibility of experiment by preventing column clogging. In addition, this product can be safely used because it does not require any organic solvents or ethanol precipitation. It can easily extract genes within 20 minutes and CAPS method is applied to the column to minimize the concerns for contamination.

Applications

Plant pathology
Rapid RNA virus detection
Virus disease monitoring
Conventional RT-PCR
Real-time RT-PCR, etc.

Kit Contents

Contents 50 Columns
Pre-Buffer 6ml × 1 ea
Lysis Buffer 50ml × 1 ea
Binding Buffer 20ml × 1 ea
Washing Buffer 120ml × 1 ea
Elution Buffer 20ml × 1 ea
Spin Columns(Orange color) 50 Columns

Technical Data

Sensitivity data for pathogenic plant virus detection

RT-PCR was performed using extracted double-RNA by Double-RNA Viral dsRNA Extraction Mini Kit(As you see above, efficiency of extraction with Double-RNA Viral dsRNA Extraction Mini Kit For Plant Tissue was higher compare to others.
iNtRON, purified RNA with Double-RNA Viral dsRNA Extraction Mini Kit; Manual; purified RNA with manual method A, potato X virus(PVX); B, potato leaf roll virus(PLRV); C, potato Y virus(PVY); D, potato S virus(PVS) Lane M, 100bp ladder; lane 1, 20 ㎕ of RNA used as template of RT-PCR; lane 2, 10 ㎕ of RNA used as template of RT-PCR); lane 3, 5 ㎕ of RNA used as template of RT-PCR; lane 4, 2.5 ㎕ of RNA used as template of
RT-PCR

Comparison data between Double RNA and total RNA (Potato X virus)

After serial dilution was performed with extracted RNA using the Double-RNA Viral dsRNA Extraction Mini Kit (For Plant Tissue) and manual method, PVX was confirmed with Maxime RT-PCR PreMix Kit (iNtRON, Cat. No. 25131) . As shown from the results, it can be seen that the extracted RNA is higher yield than the manual extracted RNA.

Lane M, 100 bp Ladder; lane N, No template control; lane 1, 0.5 ㎕ of extracted RNA used as RT-PCR template lane 2~7, 0.5 ㎕ of 1/10 serially diluted RNA used as RT-PCR template from 10-1 to 10- 6

Información adicional

Cat.

17412

Cap.

50 col.

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