DNA-spin™ Plasmid DNA Purification Kit

Spin column type of product for separation/purification up to 20ug of high purity Plasmid DNA.

En existencia

SKU: DL0014
Categoría:

Descripción

Spin column type of product for separation/purification up to 20ug of high purity Plasmid DNA

  • Up to 20ug high yield and high purity of plasmid DNA can be obtained by separation and purification with high reproducibility.
  • Separation and purification for up to 30 kb of plasmid DNA.
  • Containing Lysis & Neutralization indicator (Lysis Viewer).

 
DNA-Spin™ Plasmid DNA Purification Kit is able to perform separation and purification for high concentrated, purity plasmid DNA from bacteria using silica membrane and optimized alkali lysis buffering system. Neutralization buffering system used for pH neutralization and binding perform not only separation/purification for plasmid DNA over 30Kb, but also remove chromosomal DNA, cell debris effectively after lysis. Also, pH change through alkaline lysis method can be observed visually in real time because the product contains lysis & neutralization indicator such as Lysis Viewer (Figure 1). Product is used for either High-copy plasmid DNA or low-copy plasmid DNA and in order to perform separation and purification effectively from endA+ strain(HB101, the JM strains, NM series strains, PR series strains, etc.), Washing Buffer A is provided separately.
 
Figure1. Changes observed by Lysis Viewer

 
 

Applications

Ligation & transformation
PCR & Real-time PCR
Microinjection
Transfection
Automated fluorescent sequencing
Restriction digestion etc.

Kit Contents

Contents 50 COLUMNS 200 COLUMNS
Resuspension Buffer 15ml × 1 ea 55ml × 1 ea
Lysis Buffer 15ml × 1 ea 55ml × 1 ea
Neutralization Buffer 20ml × 1 ea 80ml × 1 ea
Washing Buffer A 30ml × 1 ea 140ml × 1 ea
Washing Buffer B(Concentration) 10ml × 1 ea 40ml × 1 ea
Elution Buffer 20ml × 1 ea 20ml × 1 ea
Spin Column & Collection tube
(Yellow color)
50 Columns & tubes 200 Columns & tubes
RNase A (Lyophilized powder) 9mg/ml × 1 tube 33mg/ml × 1 tube
Lysis Viwer 60 µl 220 µl
Manual 1 ea 1 ea

 
 

Technical Data
 
Observation based on effects of Plasmid Copy No.

After extracting plasmid DNA from high copy number and low copy number using DNA-spinTM Plasmid DNA Purification Kit, the results verified high purity and high yield of plasmid DNA.
DH5a/pUC18(High-copy number), DH5a/pET40b(Low-copy number)
iNtRON; Supplier A, Competitor A; Supplier B, Competitor B
 
 
Data Comparison for extraction efficiency based on size of plasmid DNA

After extracting various size of plasmid DNA using DNA-spinTM Plasmid DNA Purification Kit, the results verified higher yield compare to competitors.
Plasmid DNA Size : 2.7 Kb, 6.4 Kb, 9 Kb, 12.5 Kb, 33.4 Kb
NtRON; Supplier A, Competitor A; Supplier B, Competitor B

Información adicional

Cat.

17098

Cap.

200 col.

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